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题目:: Mir-33 regulates cell proliferation and cell cycle progression.
作者:: Cirera-Salinas(Daniel),Pauta(Montse),Allen(Ryan M),Salerno(Alessandro G),Ramírez(Cristina M),Chamorro-Jorganes(Aranzazu),Wanschel(Amarylis C),Lasuncion(Miguel A),Morales-Ruiz(Manuel),Suarez(Yajaira),Baldan(Ángel),Esplugues(Enric),Fernández-Hernando(Carlos)
状态:: 发布时间2012-08-21 , 更新时间 2016-10-19
期刊:: Cell Cycle
摘要:: Cholesterol metabolism is tightly regulated at the cellular level and is essential for cellular growth. microRNAs (miRNAs), a class of noncoding RNAs, have emerged as critical regulators of gene expression, acting predominantly at posttranscriptional level. Recent work from our group and others has shown that hsa-miR-33a and hsa-miR-33b, miRNAs located within intronic sequences of the Srebp genes, regulate cholesterol and fatty acid metabolism in concert with their host genes. Here, we show that hsa-miR-33 family members modulate the expression of genes involved in cell cycle regulation and cell proliferation. MiR-33 inhibits the expression of the cyclin-dependent kinase 6 (CDK6) and cyclin D1 (CCND1), thereby reducing cell proliferation and cell cycle progression. Overexpression of miR-33 induces a significant G 1 cell cycle arrest in Huh7 and A549 cell lines. Most importantly, inhibition of miR-33 expression using 2'fluoro/methoxyethyl-modified (2'F/MOE-modified) phosphorothioate backbone antisense oligonucleotides improves liver regeneration after partial hepatectomy (PH) in mice, suggesting an important role for miR-33 in regulating hepatocyte proliferation during liver regeneration. Altogether, these results suggest that Srebp/miR-33 locus may cooperate to regulate cell proliferation, cell cycle progression and may also be relevant to human liver regeneration.
语言:: eng
DOI:

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