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题目:: Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer.
作者:: Li(Dan),Zhao(Yulan),Liu(Changxing),Chen(Xiaona),Qi(Yanting),Jiang(Yue),Zou(Chao),Zhang(Xiaolong),Liu(Shunying),Wang(Xuejing),Zhao(Dan),Sun(Qiang),Zeng(Zhenbing),Dress(Andreas),Lin(Marie C),Kung(Hsiang-Fu),Rui(Hallgeir),Liu(Ling-Zhi),Mao(Feng),Jiang(Bing-Hua),Lai(Lihui)
状态:: 发布时间2011-04-04 , 更新时间 2011-04-04
期刊:: Clin Cancer Res
摘要:: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer.,The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues.,MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer.,Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets.
语言:: eng
DOI:: 10.1158/1078-0432.CCR-10-1800

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