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题目:: Transcription profiling of mouse macrophage response to hypoxia, part 2
ID:
状态:: 发布时间Jan. 1, 2005 , 更新时间 May 2, 2014
物种:: Mus musculus
摘要:: We hypothesize that the culture media collected from macrophages exposed to intermittent hypoxia will induce a greater pro-inflammatory gene profile in naïve cultured macrophages than will culture media collected from macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured for 24 hours in DMEM media obtained from 1) cells cultured with intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) media exposed to intermittent hypoxia, 3) cells cultured with sustained hypoxia (8 Torr), 4) media exposed to sustained hypoxia and 4) standard tissue culture conditions (fresh DMEM media; reference).
实验种类:: transcription profiling by array
样本量:: 13
实验设计:
: 无设计数据
数据号:: E-TIGR-91
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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