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题目:: Genotyping by array of yeast to map post-meiotic segregation across the yeast genome
ID:
状态:: 发布时间March 12, 2011 , 更新时间 June 10, 2011 , 提交时间 July 13, 2010,
物种:: Saccharomyces cerevisiae
摘要:: To map post-meiotic segregation (PMS) across the yeast genome, we genotyped the two cells resulting from the first mitotic division of the four spores of 4 tetrads of a YJM789/S96 Saccharomyces cerevisiae hybrid strain. Sporulation was induced, tetrads were dissected, spores let to germinate and the two cells coming from the first mitotic division of each spore were finally dissected. DNA from each of the eight cells in each tetrad was extracted from independent overnight cultures in rich medium and hybridized to microarrays, one array per cell. Each hybridization was used to genotype the corresponding cell and genetic differences between the two cells from the same spore revealed PMS. Therefore there are 32 hybridization files, 2 per spore and 8 per tetrad.
实验种类:: genotyping by array
样本量:: 32
实验设计:
: 无设计数据
数据号:: E-TABM-1031
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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