| GeneLibs

题目:: ChIP-chip studies of Drosophila melanogaster transcription factors HB and MED
ID:
状态:: 发布时间July 22, 2010 , 更新时间 May 2, 2014 , 提交时间 June 17, 2010,
物种:: Drosophila Melanogaster
摘要:: ChIP-chip was performed using chromatin isolated from formaldehyde crosslinked, staged embryos, and affinity purified antibodies against HB and MED. Affinity purification of the antibodies was performed with E.coli expressed His-tag fusions of the N-terminal portion of the full length protein (designated as 1, e.g. anti-HB 1 ), or the C-terminal portion of the protein (designated as 2, e.g. anti-HB 2, and anti_MED 2). The chromatin immunoprecitation and hybridization for each antibody were carried out in duplicates, and in each chromatin immunoprecitation and hybridization series, two mock IP controls and two input DNA controls were also generated. The microarray used in this study is the Affymetrix Drosophila genomic DNA tiling array, which has about 3 million oligo pairs (perfect match-mismatch), covering the whole euchromatic sequences of the fly genome at a resolution of about 35 bp.
实验种类:: ChIP-chip by array
样本量:: 3
实验设计:
: 无设计数据
数据号:: E-TABM-1021
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息