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题目:: Transcription profiling of 10 individual human cell lines to identify unique genes contributed by individual cell lines to UHRR
ID:
状态:: 发布时间Nov. 1, 2005 , 更新时间 June 4, 2014 , 提交时间 Nov. 1, 2005,
物种:: Homo sapiens
摘要:: Total RNA isolated from 10 individual human cell lines were reverse-transcribed to cDNA, labeled with Cy5 and co-hybridized with Cy3-labeled UHRR onto 43,000-spot cDNA microarrays (Stanford University). The data was analyzed using GeneTraffic software. Approximately 6000-8000 spots out of 43,000 (14-18 %) were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics in only one cell line was determined.
实验种类:: transcription profiling by array
样本量:: 0
实验设计:
: 无设计数据
数据号:: E-SMDB-2651
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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