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题目:: Transcription profiling of human fibroblasts from the stroma of squamous cell carcinoma and normal dermal fibroblasts
ID:
状态:: 发布时间Dec. 31, 2009 , 更新时间 May 2, 2014
物种:: Homo sapiens
摘要:: Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes. Epithelial-mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium including the tumor progression and spreading. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity towards normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor associated fibroblast activity that influence normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4 respectively to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in medium conditioned by cancer associated fibroblast. Presence of these growth factors was also detected in tumor samples.
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
: 无设计数据
数据号:: E-MTAB-97
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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