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题目:: GIM mTEC Aire silencing
ID:
状态:: 发布时间Aug. 31, 2011 , 更新时间 May 16, 2013
物种:: Mus musculus
摘要:: We used the TriFectaTM (IDT, Integrated DNA Technologies, Coralville, IA, USA) anti-Aire RNAi sequence (GGAUUCUCUUUAAGGACUACAAUCTAGAUUGUAGUCCUUAAAGAGAAUCCUC) in order to silencing Aire mRNA. Confluent cultures of 3.10 mTEC cell line were transfected with 10nM of anti-Aire siRNA using Hiperfect reagent (Qiagen, GmbH, Hilden, Germany) following manufacturer’s instructions. After transfection, cells were cultured during 24 h in RPMI medium as above mentioned and total RNA was extracted using the mirVana kit® (Ambion, Austin, TX, USA), which served as template for cDNA synthesis.
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
: 无设计数据
数据号:: E-MEXP-3213
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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