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题目:: Transcription profiling by array of yeast haploid Sigma1278b strains to detect Yak1-regulated genes
ID:
状态:: 发布时间June 25, 2011 , 更新时间 May 15, 2013 , 提交时间 Aug. 23, 2010,
物种:: Saccharomyces cerevisiae
摘要:: We determined and classified Yak1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of different haploid MATa yeast strains of the following genotypes:YAK1 (468=YHUM0468), yak1 (1313=YHUM1313), strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 (1313Yhc) and strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 K398R (1313KDhc). All strains were grown in duplicate in YNB medium supplemented with tryptophan at 30 degrees Celsius to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.
实验种类:: transcription profiling by array
样本量:: 8
实验设计:
: 无设计数据
数据号:: E-MEXP-2885
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