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题目:: BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse ES cells
ID:
状态:: 发布时间June 9, 2008 , 更新时间 May 2, 2014 , 提交时间 Sept. 9, 2007,
物种:: Mus musculus
摘要:: We used BAF250b-/- ES cells, two independently derived clones, at 18 and 72 hr in culture and compared them with parental cell line (wild-type) at the same time in culture (two replications each). Total RNAs were extracted using Trizol (Invitrogen) according to manufacturer protocol. 2.5 ug of total RNA samples were labeled with Cy3-CTP using a Low RNA Input Fluorescent Linear Amplification Kit (Agilent). A reference target (Cy5-CTP-labeled) was prepared from the Universal Mouse Reference RNA (Stratagene). Labeled targets were purified using an RNeasy Mini Kit (Qiagen) according to the Agilent's protocol, quantitated by a NanoDrop scanning spectrophotometer (NanoDrop Technologies), and hybridized to the NIA Mouse 44K Microarray v2.2 (whole genome 60-mer oligo; manufactured by Agilent Technologies, #014117) (Carter et al. 2005) according to the Agilent protocol (G4140-90030; Agilent 60-mer oligo microarray processing protocol - SSC Wash, v1.0). All hybridizations were carried out in the two color protocol by combining one Cy3-CTP-labeled experimental target and Cy5-CTP-labeled reference target. Microarrays were scanned on an Agilent DNA Microarray Scanner, using standard settings, including automatic PMT adjustment. Keywords: genetic modification design,time series design Biological replications were performed on BAF250b-/- ES cells with their parental line (wild-type) at 18 and 72 hrs after passaging, as well as with 12 other pluripotent cells (ES and EG cells from strains C57BL/6 and 129 data from Sharova et al., 2007) using whole-genome mouse microarrays (NIA 44k V2.2 with 60-mer synthesized oligos).
实验种类:: transcription profiling by array
样本量:: 24
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数据号:: E-GEOD-8996, GSE8996
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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