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题目:: Transcription profiling of human human prostate adenocarcinoma cells following irradiation to a dose of 10 Sv from a Cesium-137 gamma source
ID:
状态:: 发布时间Nov. 8, 2007 , 更新时间 June 10, 2011 , 提交时间 Oct. 22, 2003,
物种:: Homo sapiens
摘要:: The experiment was a time course experiment on LNCaP C4-2 human prostate adenocarcinoma cells following irradiation to a dose of 10 Sv from a Cesium-137 gamma source. Total RNA was extracted from cells at 1, 2, 4, 6, 8, 12, 16, 20 and 24 hours after irradiation. The untreated control sample, labeled 0, was collected concurrently with the cells extracted at 24 hr. After extraction, the samples were processed and hybridized to the Affymetrix (Santa Clara, CA, www.affymetrix.com) HG-U95Av2 chip, and then washed, stained and scanned according to Affymetrix's protocols contained in the GeneChip(R) Expression Analysis Manual. Transcript abundance data from the scans was processed initially with Affymetrix' Microarray Suite 5(R), then by Silicon Genetics' (Redwood City, CA, www.silicongenetics.com) GeneSpring(R).
实验种类:: transcription profiling by array
样本量:: 9
实验设计:
: 无设计数据
数据号:: E-GEOD-770, GSE770, GDS725
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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