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题目:: Transcription profiling of Arabidopsis loss of function mutant AtLEJ1
ID:
状态:: 发布时间Jan. 13, 2009 , 更新时间 June 10, 2011 , 提交时间 Aug. 3, 2006,
物种:: Arabidopsis thaliana
摘要:: The loss-of-function mutant of AtLEJ1 had well-developed anther cells and showed an increased accumulation of JA and ET in its flower bud clusters. Conversely, overexpression of AtLEJ1 caused a delay in the development of the floral organs, arrested anther cell development and secondary cell-wall biosynthesis in the endothecium cells, and reduced JA and ET contents in the flower bud clusters, resulting in male-sterility through anther indehiscence. We used microarrays to gain a comprehensive understanding of the transcriptional changes induced by AtLEJ1 gene expression, Experiment Overall Design: We performed a full genome microarray analysis (ATH1 Affymetrix chips) with the wild type, 35S:AtLEJ1/5-8 line and atlej1 mutant. Total RNAs were obtained separately from unopened flower buds at stages 1~12, when the anthers are not yet opened, and from open flowers at stages 13~14, when anthesis occurs.
实验种类:: transcription profiling by array
样本量:: 18
实验设计:
: 无设计数据
数据号:: E-GEOD-5449, GSE5449
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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