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题目:: Transcription profiling of mouse CD4+ T cells responding to transient or persistent antigen presented by dendritic cells in vivo
ID:
状态:: 发布时间June 13, 2008 , 更新时间 May 2, 2014 , 提交时间 July 6, 2006,
物种:: Mus musculus
摘要:: These experiments were done to compare the gene expression profiles in CD4+ T cells responding to antigen presented by dendritic cells transiently or persistently. Some treatments include the activation of the dendritic cells by CD40 engagement. Experiment Overall Design: 2-4x106 lymph node cells from AND TCR-transgenic animals that expressed the congenic marker CD90.1 were transferred into double-transgenic (Ii-rTA/TIM) recipient animals that expressed the corresponding peptide from moth cytochrome c under a doxycycline-controllable promoter and were FACS-sorted 60 hours later. Recipients expressed the antigen not at all (“ctrl”), transiently (“short”) or throughout the experiment (“long”) due to different doxycycline treatment protocols. A second variable tested was the activation status of the dendritic cells: recipients were either injected by the stimulatory antibody FGK45.5 targeting CD40 (“FGK”) or PBS (“PBS”).
实验种类:: transcription profiling by array
样本量:: 16
实验设计:
: 无设计数据
数据号:: E-GEOD-5245, GSE5245
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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