Transcription profiling of human treated cord blood-derived human mast cells control and TGF-beta treated
ID:
状态:
发布时间June 13, 2008
, 更新时间 May 3, 2014
, 提交时间 May 23, 2006,
物种:
Homo sapiens
摘要:
Cord blood-derived stem cells were in vitro cultured in the presence of 40 ng/ml SCF, 20 ng/ml IL6 and 2 microM lysophosphatidic acid for 5 week. Then mast cells were magnetically isolated and further cultured for 4 days in the presence or absence of 2 ng/ml TGF-betaI. Total RNA was isolated and processed according to the Agilent Low-input RNA Linear Amplification Kit and microarray hybridization protocol. Experiment Overall Design: 5 biological replicates from 5 donors and one technical replicate (dye-swap)