Transcription profiling of mouse classically activated and type II activated macrophages to identify novel markers
ID:
状态:
发布时间June 13, 2008
, 更新时间 March 27, 2012
, 提交时间 May 11, 2006,
物种:
Mus musculus
摘要:
The purpose of this study is to identify novel markers for the type II activated macrophage, which is generated by classical stimulation in the presence if IgG immune complexes. These cells gererally produce high levels of IL-10 and low levels of IL-12, in comparison to classically activated macrophages. We wish to identify gene expression which is enriched in Type II activated macrophages in comparison to classically activated macrophages. Experiment Overall Design: The design of this experiment is to simulateously stimulate two popultions of macrophages and compare their gene expression. In this case, macrophages are primed overnight with IFN-gamma, washed, then stimulated with LPS (Classically) or LPS+Immune complexes (Type II).