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题目:: Transcription profiling of placenta from pregnant C57/BL mice treated with dexamethasone (0.5 mg/kg) on gestational day 15, 16, 17, vs. saline treated controls to examine the effect of antenatal steroids on fetal growth
ID:
状态:: 发布时间Dec. 7, 2007 , 更新时间 June 10, 2011 , 提交时间 Feb. 2, 2006,
物种:: Mus musculus
摘要:: Antenatal steroids (AS) are used to augment fetal lung maturity and there is a major concern that they impair fetal growth. If delivery is delayed after using AS, placental function and hence fetal growth may be compromised even further. We treated three pairs of pregnant C57/BL mice with AS and studied changes in placental gene expression by microarray analysis, these results were later confirmed by semi quantitative RT-PCR, in situ hybridization and Oligo ApopTaq assay. AS treated placentas were hydropic, friable and pale. Placental weight was decreased (78.8±16.7 mg compared to 81.3±17 mg, p<0.05) and so was fetal weight (914 38 mg compared to 1160 85 mg, p<0.05). There was more than 99% similarity between the three gene chip data sets. AS led to down-regulation of 1212 genes and up-regulation of 1382 genes. AS led to decrease in expression of genes involved in cell division like cyclin A2, B1, D2, CDK2, CDK4 and M phase protein kinase along with growth promoting genes like BMP4 and IGF-BP4. Tryptophan hydroxylase gene a key regulator of serotonin production was upegulated by >15 fold. AS treatment caused deleterious effects on placenta by decreasing expression of growth related genes and increased apoptosis of trophoblast cells. 3 sets of pregnant C57/BL mice were treated with dexamethasone (0.5 mg/kg) on gestational day 15, 16, 17. 3 sets of pregant mice were injected with saline and served as controls.
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-4165, GDS2314, GSE4165
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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