Expression data from HeLa cells transiently transfected with control siRNA or SON siRNA
ID:
状态:
发布时间April 1, 2011
, 更新时间 April 14, 2011
物种:
Homo sapiens
摘要:
SON is a large Ser/Arg (SR)-related protein localized in nuclear speckles. SON siRNA causes defects in mitotic progression and genome instability. We used microarrays to detail the pattern of gene expression after SON knockdown. HeLa cells were grown in 100 mm dishes and transfected with negative control siRNA or SON siRNA (400 pmol). Then, cells were harvested after 66 hours and RNAs were isolated. Microarrays were performed using GeneChip Human Genome U133A 2.0 (Affymetrix).