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题目:: Drop-on-demand single cell isolation and total RNA analysis
ID:
状态:: 发布时间Oct. 1, 2010 , 更新时间 June 10, 2011 , 提交时间 Sept. 22, 2010,
物种:: Mus musculus
摘要:: We present a microdroplet-based method to isolate and pattern single cells from heterogeneous cell suspensions (10% target cell mixture), preserve viability of the extracted cells (97.0 ± 0.8%), and obtain genomic information from isolated cells compared to the non-patterned controls. To evaluate the sensitivity and reproducibility of a functional genomic analysis of stem cell encapsulating droplets, we used DNA microarrays to measure gene expression levels in RNA samples extracted from mouse embryonic stem cells obtained by using drop-on-demand. We compared the results with RNA extracted from a control pool of mESCs, which were isolated with serial dilution and manual pipetting.
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-24330, GSE24330
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