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题目:: KIM5 intracellular expression profiling
ID:
状态:: 发布时间July 7, 2010 , 更新时间 May 2, 2014
物种:: Yersinia pestis
摘要:: Microarrays were used to determine the transcriptional profile of Y. pestis that is growing inside macrophages. J774A.1 macrophage-like cells were infected with Y. pestis KIM5 and incubated in the presence of gentamicin in tissue culture media. RNA was isolated from intracellular bacteria at various time points post infection. Control bacteria were grown for 4 hours in tissue culture medium under the same conditions without macrophages or gentamicin. The transcriptional profiles of intracellular Y. pestis at different time points were compared to those of control Y. pestis using the 70-mer oligonucleotide microarrays obtained from Pathogen Functional Genomics Resource Center/J. Craig Venter Institute (Y. pestis microarray version 2). RNA from samples of Yersinia pestis KIM5 at 3 time points in were compared to 12 Yersinia pestis in DMEM for 4 hours.
实验种类:: transcription profiling by array
样本量:: 24
实验设计:
: 无设计数据
数据号:: E-GEOD-22168, GSE22168
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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