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题目:: Gene expression profiles of an E10.5 ureteric duct cell (GUDMAP Series_id: 40)
ID:
状态:: 发布时间May 27, 2010 , 更新时间 June 10, 2011 , 提交时间 April 28, 2010,
物种:: Mus musculus
摘要:: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. HoxB7-GFP transgenic mice were utilized to isolate the ureteric duct cells from E10.5 embryonic kidneys. The ureteric duct cells were isolated from embryos using microdissection, trypsin treatment and FACS. The RNA was isolated from purified ureteric duct cells and the gene expression profiles were determined by microarrays.
实验种类:: transcription profiling by array
样本量:: 3
实验设计:
: 无设计数据
数据号:: E-GEOD-21584, GSE21584
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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