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题目:: Molecular Hydrogen Impact on Chalcopyrite Bioleaching by the Extremely Thermoacidophilic Archaeon Metallosphaera sedula
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状态:: 发布时间May 5, 2010 , 更新时间 May 1, 2014 , 提交时间 Feb. 27, 2010,
物种:: Metallosphaera sedula
摘要:: Hydrogen served as a competitive inorganic energy source, impacting the CuFeS2 bioleaching efficiency of the extremely thermoacidophilic archaeon Metallosphaera sedula. Open reading frames encoding key terminal oxidase and electron transport chain components were triggered by CuFeS2. Evidence of heterotrophic metabolism was noted after extended periods of bioleaching, presumably related to cell lysis. One 3 slide loop for Mse cells includes 3 conditions: M. sedula inoculum prior to introduction to chalcopyrite (d0~day 0), M. sedula after 3 days on chalcopyrite (d3~day 3), and M. sedula after 9 days on chalcopyrite (d9~day 9). Half of an RNA sample for one condition (consisting of pools of RNA from multiple cultures) was labeled with Cy3 while the other half was labeled with Cy5. The two differently labeled samples were run on different slides. Each probe is spotted on each slide 5 times (5 spots/slide x 2 slides = 10 technical replicates per condition; spot intensities for all replicates on slide provided in associated raw data file).
实验种类:: transcription profiling by array
样本量:: 6
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数据号:: E-GEOD-20554, GSE20554
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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