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题目:: ArrayCGH of Trichoderma reesei strains QM9123, QM9414, NG14 and RutC-30
ID:
状态:: 发布时间Nov. 26, 2010 , 更新时间 May 1, 2014
物种:: Hypocrea jecorina
摘要:: Trichoderma reesei is the main industrial producer of cellulases and hemicellulases used to depolymerize biomass in many biotechnical applications. Many production strains in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in hyperproducing mutants of T. reesei by high-resolution comparative genomic hybridisation tiling array. We carried out aCGH analysis of four hyperproducing strains (QM9123, QM9414, NG14 and RutC-30) using QM6a genome as a reference. ArrayCGH analysis identified dozens of mutations in each strain analyzed. 2.1 million oligonucleotide probe custom aCGH (HD2 format, RocheNimblegen) was designed according to T. reesei strain QM6a genome v2.0 (
实验种类:: comparative genomic hybridization by array
样本量:: 28
实验设计:
: 无设计数据
数据号:: E-GEOD-19606, GSE19606
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