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题目:: SoxC ChIP-chip on Ciona intestinalis embryo (early gastrula)
ID:
状态:: 发布时间May 6, 2010 , 更新时间 May 1, 2014 , 提交时间 Aug. 16, 2009,
物种:: Ciona intestinalis
摘要:: A genomic overview of in vivo binding of a transcription factor SoxC in the ascidian early gastrula embryo. Fertilized eggs were electroporated with a construct encoding GFP-tagged SoxC. After fixation, chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE (Whole Cell Extract) DNA were amplified by LM-PCR for the Chip analysis. Two independent experiments were performed to reduce experimental errors.
实验种类:: ChIP-chip by tiling array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-17692, GSE17692
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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