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题目:: Transcription profiling of porcine olfactory bulb neuroblasts and PK15 non-neuronal, epithelial origin cell cultures
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状态:: 发布时间Nov. 10, 2009 , 更新时间 May 2, 2014 , 提交时间 June 26, 2009,
物种:: Sus scrofa
摘要:: The phenotypically characterized hTERT immortalized porcine olfactory bulb neuroblast cell line (OBGF400) was subjected to an extensive whole genome-scaled expression profile for establishing their use as an in vitro neuronal disease model system. Microarrays were used to provide a comprehensive knowledge underlying the genomic complexity and overall gene expression capacity of the immortalized OBGF400 cells. The analysis revealed the elaborate signaling mechanisms of this unique subpopulation of porcine neuronally committed progenitors that mirrors the intricate organization of postnatal neurongenic zones. SUBMITTER_CITATION: Transcriptome Profile and Cytogenetic Analysis of Immortalized Neuronally Restricted Progenitor Cells Derived from the Porcine Olfactory Bulb. Animal biotechnology 2009 vol:20 iss:4 page:186-215 Experiment Overall Design: Total cellular RNA extracts from independent OBGF400 (neuroblasts) and PK15 (non-neuronal, epithelial origin) cell cultures (9 each) were pooled into a total of 3 biological replicates per cell line. The concentrations and purity of the pooled RNA preparations were determined using the BioAgilent RNA assay prior to hybridization on Affymetrix GeneChip® Porcine Genome Expression Arrays. To ascertain the genes that were preferentially expressed by the OBGF400 neuroblasts, we used the PK15 cellular array in an effort to exclude somatic cell background.
实验种类:: transcription profiling by array
样本量:: 6
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数据号:: E-GEOD-16855, GSE16855
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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