Differences between CD44+/CD24- and CD44-/CD24+ subpopulation of immortalized human mammary epithelial cells
ID:
状态:
发布时间March 7, 2010
, 更新时间 May 4, 2014
, 提交时间 March 11, 2009,
物种:
Homo sapiens
摘要:
CD44+/CD24- subpopulation of normal and cancerous breast epithelial cells are suggested to have stem cell properties. The goal of this study was to identify gene expression differences between CD44+/CD24- and CD44-/CD24+ subpopulation of cells from a same cell lines. We selected MCF-10A cells, which are immortalized derived from a fibrocystic breast disease. These cells are immortalized but not transformed and express basal cell markers. Cells were from a single sort but plated into four 100 mm plates. RNA was prepared from each plate separately for the analysis. Comparison of gene expression between 2 groups ( CD44+/CD24- and CD44-/CD24+) 4 replicates each.