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题目:: Evolution of E. coli transcriptome under osmotic-stress with and without RpoS
ID:
状态:: 发布时间Nov. 9, 2009 , 更新时间 March 27, 2012 , 提交时间 Nov. 19, 2008,
物种:: Escherichia coli
摘要:: The RpoS sigma factor protein of Escherichia coli is the master transcriptional regulator of physiological responses to a variety of stresses. This stress response comes at the expense of scavenging for scarce resources, causing a trade-off between stress tolerance and nutrient acquisition. This trade-off favors non-functional rpoS alleles in nutrient-poor environments. We used experimental evolution to explore how natural selection modifies the regulatory network of strains lacking RpoS when they are evolved in an osmotically stressful environment. We found that strains lacking RpoS adapt less variably, in terms of both fitness increase and changes in patterns of transcription, than strains with functional RpoS. This phenotypic uniformity was caused by the same adaptive mutation in every independent population: the insertion of IS10 into the promoter of otsBA. OtsA and OtsB are required to synthesize the osmoprotectant trehalose, and transcription of otsBA requires RpoS in the wild-type genetic background. The evolved IS10 insertion rewires expression of otsBA from RpoS-dependent to RpoS-independent, allowing for partial restoration of wild-type response to osmotic stress. Our results show that the regulatory networks of bacteria Keywords: evolution; expression This study started with wild-type and rpoS- strains, and evolved five lines from each, for a total of 12 strains. Expression was measured for each strain with two separate biological replicates of RNA
实验种类:: transcription profiling by array
样本量:: 24
实验设计:
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数据号:: E-GEOD-13666, GSE13666
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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