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题目:: Transcription profiling of mouse activated or control 5-FU bone marrow from MLL-AF4stop knockins
ID:
状态:: 发布时间Nov. 13, 2008 , 更新时间 March 27, 2012 , 提交时间 July 31, 2008,
物种:: Mus musculus
摘要:: We created a mouse model where conditional expression of physiologic levels of an Mll-AF4 fusion oncogene induces development of acute lymphoblastic (ALL) or acute myeloid leukemias (AML). Immunophenotypic and gene expression analysis of the ALL cells demonstrated bone marrow replacement with B-precursor cells which express a gene expression profile that has significant overlap with profiles in human MLL-rearranged ALL. To examine early, pre-leukemic changes in lymphoid cells due to Mll-AF4 expression, we infected 5-FU bone marrow cells from Mll-AF4stop knocking mice with activating (Cre-GFP) or control (MIF-GFP) retrovirus ex vivo and measured expression changes after culture under lymphoid growth conditions. Experiment Overall Design: Mll-AF4stop knock-in mice were treated with 5-FU and 5 days later their bone marrow infected ex vivo with either Cre-GFP to activate the Mll-AF4 fusion construct or with a control MIG-Cre retrovirus. GFP+ cells were sorted 2 days post-infection and cultured for 14 days under lymphoid growth conditions before total RNA was isolated for hybridization to Affymetrix expression microarrays.
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
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数据号:: E-GEOD-12313, GSE12313
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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