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题目:: Global Transcriptome Profiles in the Nodes of Rice During Spatial and Temporal Development
ID:
状态:: 发布时间June 26, 2008 , 更新时间 May 1, 2014 , 提交时间 June 25, 2008,
物种:: Oryza sativa
摘要:: The following study was conducted to better understand the genetics of the spatial and temporal development of rice, especially as this relates to the development of lignocellulosic tissues. To do so, an oligonucleotide array was designed and created by the Plant Sciences Institute at Iowa State University. A total of 43,776 spots were printed on two separate platforms, the first contained 23,040 spots while the second contained 20,736. RNA was collected from nodes of rice seedlings at several different time points, as nodes are highly lignified tissues. Comparisons were performed to identify those genes exhibiting differential regulation across time at an anatomical position as well as across anatomical distance. For example, node 1 was compared across days 46, 53, 60, and 67 and at day 67 node 1 was compared to nodes 2, 3, and 4. Similar comparisons were performed for nodes 2, 3, and 4 where applicable. Keywords: Time course and anatomical development in rice For each comparison, 6 replicates and multiple power settings were considered. Dye-swaps were performed between replicates. A diagram of the comparisons made in this experiment may be found in ISU_RiceArray_LoopDesign.pdf (linked below as a Supplementary file).
实验种类:: transcription profiling by array
样本量:: 268
实验设计:
: 无设计数据
数据号:: E-GEOD-11896, GSE11896
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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