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题目:: Histone deacetylase 4 represses dystrophin-glycoprotein (DGC) complex expression
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状态:: 发布时间May 25, 2010 , 更新时间 May 2, 2014 , 提交时间 Feb. 29, 2008,
物种:: Mus musculus
摘要:: Dysfunction of the dystrophin-glycoprotein complex (DGC) is a frequent cause of hereditary forms of muscular dystrophy. Although DGC function in maintaining skeletal muscle integrity has been well characterized, little is known about how the DGC complex is coordinately regulated at the transcriptional level. To test this hypothesis, we engineered HDAC4 stably overexpressing and control myotubes in an in vitro model of muscle differentiation. Here we present evidence that HDAC4, a neural activity-responsive histone deacetylase, is a critical transcriptional regulator of the DGC complex. We show that HDAC4 can repress multiple components of the DGC complex, including dystrophin and sarcoglycan family members in both cultured myotubes. To confirm this finding, the protein levels of core DGC complex members including dystrophin, sarcoglycan complex members, and additional dystrophin-associated proteins were evaluated in differentiated myotubes by western analysis. C2C12 mouse myotubes were infected with either a HDAC4 expressing or control (Neo) retroviruses. After stable selection, myotubes were differentiated at 90% confluency in 2% horse serum (Hyclone) for 4 days. At this time point, RNA was extracted and the two types of cells compared in a microarray analysis.
实验种类:: unknown experiment type
样本量:: 16
实验设计:
: 无设计数据
数据号:: E-GEOD-10678, GSE10678
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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