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题目:: Transcriptional Analysis of Medicago truncatula and Glycine max Using Tiling Microarrays
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状态:: 发布时间May 26, 2010 , 更新时间 March 27, 2012 , 提交时间 Jan. 11, 2008,
物种:: Glycine max, Medicago truncatula
摘要:: This SuperSeries is composed of the following subset Series: GSE10055: Transcriptional Analysis of 1M Regions in Medicago truncatula Using Tiling Microarrays GSE10056: Transcriptional Analysis of 1M Regions in Glycine max Using Tiling Microarrays Keywords: tiling array, transcriptional analysis Both the barrel medic and soybean tiling arrays were produced on the Maskless Array Synthesizer platform. Briefly, tiling-paths consisting of 36-mer oligonucleotides offset by five nucleotides were designed to represent both DNA strands of the selected barrel medic and soybean genome sequence. Probes were synthesized at a feature-density of 390,000 probes per array in a “chess board” design. Microarray production and storage were carried out. Total RNA and mRNA were sequentially isolated using the RNeasy Plant Mini kit (Qiagen, Valencia, CA) and the Oligotex mRNA kit (Qiagen) according to the manufacturer’s recommendations, respectively. mRNA from different organ types was reverse transcribed using a mixture of oligo(dT)18 and random nonamer primers, during which amino-allyl-modified dUTP (aa-dUTP) was incoporated. The aa-dUTP decorated cDNA was fluorescent labeled by conjugating the monofunctional Cy3 dye (GE Healthcare, Piscataway, NJ) to the amino-allyl functional groups in the cDNA. Two μg dye-labeled targets were used for hybridization.
实验种类:: transcription profiling by tiling array
样本量:: 12
实验设计:
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数据号:: E-GEOD-10151, GSE10151
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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